Application Notes & Technical Insights

📘 Fluorescence Imaging with Axiocam 807 Mono

The Axiocam 807 Mono camera provides exceptional sensitivity for low-light fluorescence applications. To maximize performance:

• Use low excitation intensity to reduce phototoxic effects.
• Activate the cooling system to reduce dark current noise during long exposures.
• Apply flat-field correction in software to eliminate uneven illumination artifacts.

🔍 Image Stitching for Large Field of View

For biological and materials research, stitching allows you to observe wide samples with full detail. Recommendations include:

• Use a motorized stage for precise tile acquisition.
• Maintain 10–15% overlap between adjacent fields.
• Employ ZEN software for stitching with shading correction enabled.

🔬 Live Cell Imaging Guidelines

Live cell workflows demand environmental control and sensitivity to prevent cell stress:

• Use incubation chambers to maintain 37°C, 5% CO₂, and humidity.
• Utilize minimal exposure times, even in time-lapse sequences.
• Optimize autofocusing methods to reduce sample disturbance.